FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445982
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG29160.6538380472754506No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT28370.6361243278876726No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG24970.5598880672314129No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC21780.48836051679215753No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA21160.47445861043719256No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA19870.44553367624702345No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG19230.4311833212999628No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT17170.38499311631411137No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG15930.35718930360418133No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG14580.3269190236377253No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT14440.3237798834930558No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG14160.31750160320371673No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT13930.31234444439461684No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA13910.3118959958025212No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA12800.2870070989412129No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12510.28050459435582603No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12320.2762443327309174No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG12100.2713113982178653No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG11970.26839648236924357No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG11820.26503311792852624No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG11620.26054863200756984No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG11150.25001009009332215No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC10620.23812620240278753No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG10400.23319326788973546No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT10310.23117524922530505No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG10110.2266907633043486No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG10030.22489696893596606No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG9960.22332739886363126No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9860.22108515590315306No Hit
TTCGTCGGTCTCTTCCAGACGACGACCCGGAGACATACGGGCAG9560.21435842702171837No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG9140.20494100658770983No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG9100.2040441094035185No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC8960.20090496925884901No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT8720.19552358615370127No Hit
CATACGGTCCAGGTCTTCGGTCGCGGAGCGAGCACGGGTGCTGG8700.1950751375616056No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG8140.18251857698292756No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG8140.18251857698292756No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT8020.1798278854303537No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8020.1798278854303537No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG7970.1787067639501146No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG7870.17646452098963636No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC7610.17063468929239298No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT7190.16121726885838442No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG7150.16032037167419314No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG6730.15090295124018457No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG6720.15067872694413675No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6640.1488849325757542No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6610.1482122596876107No Hit
CTTTGGGTGTTTCTGTTTCTGACGCGCTTCGCTAGAGGTACGTT6560.1470911382073716No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6550.14686691391132378No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG6380.1430551008785108No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA6280.14081285791803258No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC6200.13901906354965No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG6180.13857061495755435No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA6150.13789794206941086No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT5770.1293774188195936No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5750.12892897022749797No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG5710.1280320730433067No Hit
GGTATCGGAAGAGTCGTCATCCTCTTCTTCGGAGTCTTCTACCG5650.12668672726701974No Hit
TACCTGGATGTCCAGATCGAAGTAGAAACGGCGTGCCATGGCCG5480.12287491423420677No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG5350.11995999838558508No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG5110.11457861528043733No Hit
AACCGTGGATTCCGCCTTCAGGCGGCCACGGGTCATAACACCCG5070.11368171809624603No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA5040.11300904520810257No Hit
TTTGGAGGTGAACGGTTTACCTTTATCCGGCAGGTCTGCCATCG4960.11121525083971999No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG4920.1103183536555287No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT4910.11009412935948089No Hit
TTCTGCGGAGTTCGGTACGATTTCCAGCTGCGGAACTTTATATT4880.10942145647133741No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA4850.10874878358319395No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG4850.10874878358319395No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT4830.10830033499109828No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG4750.10650654062271572No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG4740.10628231632666789No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA4660.10448852195828531No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG4630.10381584907014185No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG4610.10336740047804618No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG4540.10179783040571144No Hit
ACCGTGGTCGCCGTTGTCGCCATCGTCACCATCGTCGCCGTCAT4490.10067670892547231No Hit
CAGACCCATTGGTTCGTTCATGATGTCGTTGTCCAGGCCCGGAC4460.10000403603732885No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)