FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007926518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445982
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63491.4236000556076256No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT51091.1455619285083254No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29360.6583225331964071No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG28750.6446448511374898No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG27340.6130292253947469No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG24140.5412774506594437No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG18270.4096577888793718No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG17810.3993434712611719No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC17160.3847688920180635No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT16520.37041853707100286No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG16020.35920732226861174No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG16010.3589830979725639No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG15190.34059670569664247No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15120.3390271356243077No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA13620.3053934912171343No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13600.3049450426250387No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13470.302030126776417No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12960.290594687677978No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC12900.2892493419016911No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12700.28476485598073464No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC12640.2834195102044477No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG11730.26301509926409583No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA11610.260324407711522No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11520.2583063890470916No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC11370.25494302460637425No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10420.23364171648183113No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC10170.22803610908063554No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10140.22736343619249205No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9970.2235516231596791No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9880.2215336044952487No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9820.22018825871896178No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9590.21503109990986183No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC9570.2145826513177662No Hit
ACCCTGGAACCGGTATCCCGCATTCGTTCTC9220.20673480095609237No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG8970.20112919355489683No Hit
ATGTCTTCTCGCCGCCGTAGCTCTTCCCGTC8830.1979900534102273No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA8700.1950751375616056No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8530.19126332452879263No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8510.190814875936697No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8390.18812418438412312No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC8250.1849850442394536No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7990.17915521254221023No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7980.17893098824616238No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7790.17467072662125377No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC7330.16435640900305393No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7280.16323528752281483No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7250.16256261463467137No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7180.1609930445623366No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA7160.16054459597024096No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG6730.15090295124018457No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6650.149109156871802No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG6620.14843648398365855No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6570.14731536250341942No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6470.1450731195429412No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT6460.14484889524689337No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6300.1412613065101282No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC6170.13834639066150653No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT5810.13027431600378492No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG5690.12758362445121105No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5610.12578983008282846No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5600.12556560578678064No Hit
GTGCCATTCATTCCGCCGATGGCGGATCTGC5590.12534138149073282No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5330.11951154979348944No Hit
TCCATCTCTAGCAGCGAGGAAATCGTCCCAA5270.1181662040172025No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG5180.11614818535277209No Hit
GAACTGGTGACCACCGAACGCAAAACTCCGC5090.1141301666883417No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG5090.1141301666883417No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5060.11345749380019822No Hit
TACTTCGATGGTTATGACCAGCAGTCTGTAG5010.1123363723199591No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5010.1123363723199591No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG4990.11188792372786346No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4910.11009412935948089No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4910.11009412935948089No Hit
TGGATCGAAGTATCCGGCCAGGAGCTGCCGA4780.10717921351085918No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG4640.10404007336618966No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG4500.10090093322152015No Hit
ATTGAAGTTATCGACGTTGAAACCACTGAAG4480.10045248462942451No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)