FastQCFastQC Report
Sun 21 May 2023
EGAF00007926540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424015
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT57301.3513672865346744No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC33830.7978491326957773No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG22250.5247455868306545No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA21710.5120101883188095No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21310.5025765597915168No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19130.45116328431777175No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG17710.41767390304588276No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG15300.3608362911689445No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15000.353761069773475No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC13900.32781859132342017No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG13180.31083805997429337No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13150.3101305378347464No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC12180.28725398865606167No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10110.23843496102732212No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9920.2339539874768581No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9920.2339539874768581No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9700.22876549178684719No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC9690.22852965107366482No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC9210.21720929684091364No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9070.21390752685636122No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8860.20895487187953254No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG8840.20848319045316793No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8620.20329469476315695No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8600.2028230133367923No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8370.19739867693359903No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7230.17051283563081493No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6970.16438097708807473No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6490.1530606228553235No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6400.15093805643668265No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6170.1455137200334894No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5910.13938186149074916No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG5880.13867433935120219No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5790.13655177293256135No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5700.13442920651392049No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5270.12428805584708089No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5070.11957124158343455No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG4790.11296770161432969No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT4570.10777920592431871No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG4430.10447743593976627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)