FastQCFastQC Report
Sun 21 May 2023
EGAF00007926730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498364
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21450.43040829594432983No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG15150.30399467056207913No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT14340.28774149015578976No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14330.28754083360756394No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG13790.276705380003371No Hit
GCGAACGAAGCAGGCCAGGTGGCCGGCGGTG13600.27289290558708096No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG13210.26506730020627495No Hit
TCCTGCGATCGTTGCTGTCCGGGCTACCACC12890.2586462906630495No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC12450.2498174025411145No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11930.2393832620333732No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG11880.23837997929224425No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11790.23657407035821207No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA11260.22593927330224495No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11250.22573861675401916No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG11090.22252811198240643No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC10660.21389988040869723No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG10580.21229462802289087No Hit
CCGGAAAAACTGCCGAAAACTATCGAGAAAC10100.20266311370805276No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG9090.18239680233724748No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9030.1811928630478927No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG8930.17918629756563476No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8770.17597579279402203No Hit
CTGAACCTGCCGGAAGAAGTAGTGCAGTACA8630.1731666011188609No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8350.16754821776853868No Hit
GGTTGCCCGCTGCCGCCGCCGCGTTCCCCGC7900.1585186730983779No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC7790.15631145106789415No Hit
GGCATTGACGAGAACGTCTGCACTATGAAAC7660.15370291594095883No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT7190.14427205817434646No Hit
AAAGAAGTAGTCTGTGCGAGCTCCAGCGACA7080.1420648361438627No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6900.13845301827579842No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC6630.13303529147370197No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6330.1270155950269281No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG6280.12601231228579912No Hit
CGTAAACTGCACGAATGGGAAAGCCGTACCA6180.12400574680354118No Hit
CTGGACAGCCTGCGCATGGAGATGGAACGTC6110.12260115096596061No Hit
GCGACCGGCCTGGAAGACCAGGCAGAACGTA5930.11898933309789632No Hit
GGTTACCAGAAACTGCGTCATAACATCGACA5850.11738408071208996No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC5700.11437423248870304No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG5680.11397291939225145No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5480.10995978842773554No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5460.10955847533128396No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5460.10955847533128396No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG5290.10614731401144543No Hit
GGCTACACTTCCGGCCGTAACCTGTACACCA5070.10173286995047796No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG5070.10173286995047796No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG5060.10153221340225217No Hit
ATGTCCGACACCAAAACCAGCACTGGTTCTA5020.10072958720934899No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG5000.10032827411289741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)