FastQCFastQC Report
Wed 31 May 2023
EGAF00007926738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515314
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA78111.5157748479567796No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC48780.94660731127041No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA40380.7835999021955544No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA35190.682884610160019No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA27190.5276394586601567No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT26070.5059051374501761No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG23630.458555366242718No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT23010.4465238670014787No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG17850.3463907442840676No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA17780.3450323492084438No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG17470.3390165995878241No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16880.3275672696647093No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC16400.3182525605747175No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG12090.23461423520416677No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12010.23306178368916816No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC11840.22976282421979607No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11560.2243292439173009No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11510.22335896172042674No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT11040.21423830906980987No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10210.19813162460169914No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9990.19386238293545294No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9820.19056342346608088No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9300.18047248861858983No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG8620.16727665074110154No Hit
TCCTACACCAACGGCAAACTGACTATCACCC8560.16611231210485258No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8340.16184307043860638No Hit
ATGGCAATTAAACACACCACTATCGACATCG8040.15602137725736154No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7990.1550510950604874No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7960.1544689257423629No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7580.14709478104611945No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7120.13816818483487736No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7000.13583950756237945No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6890.13370488672925634No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6800.1319583787748829No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT6710.13021187082050942No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6690.12982375794175977No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6530.12671885491176255No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA6500.12613668559363805No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA6420.12458423407863944No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6170.11973282309426873No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6170.11973282309426873No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC6070.11779225870052046No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5950.11546358142802253No Hit
ACTACCGACCCGGATGCAGCGGCTTCCGCGA5240.10168557423240975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)