FastQCFastQC Report
Sun 21 May 2023
EGAF00007926740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007926740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518600
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA80431.550906286155033No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC49200.9487080601619745No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA39400.759737755495565No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA35220.6791361357500963No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA28170.5431932124951794No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT25510.49190127265715383No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG24320.46895487851908985No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT22490.4336675665252603No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA19240.37099884303895103No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC18070.3484381025838797No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG17550.3384111068260702No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG17490.3372541457770922No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC14920.28769764751253374No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC12110.23351330505206325No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG11660.2248360971847281No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG11620.22406478981874278No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT11610.22387196297724643No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11550.22271500192826843No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC11010.21230235248746626No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA10590.20420362514462012No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10440.2013112225221751No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10420.20092556883918242No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10420.20092556883918242No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8840.17045892788276126No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8480.16351716158889318No Hit
TCCTACACCAACGGCAAACTGACTATCACCC8430.16255302738141147No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG8220.15850366370998842No Hit
ATGGCAATTAAACACACCACTATCGACATCG8140.15696104897801774No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7990.15406864635557269No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7450.14365599691477055No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7250.1397994600848438No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA7220.1392209795603548No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6940.13382182799845738No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6820.13150790590050135No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA6820.13150790590050135No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6790.13092942537601235No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6630.12784419591207097No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT6530.1259159274971076No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6500.1253374469726186No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC6350.12244504435017356No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6080.11723871962977246No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5750.11087543386039338No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5450.10509062861550328No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC5320.1025838796760509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)