FastQCFastQC Report
Sun 21 May 2023
EGAF00007927024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499923
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85161.7034623331993126No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT58111.1623790063669805No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC24700.4940760877175085No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21980.4396677088271594No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC20060.4012617943163247No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG19650.39306053132182356No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19520.39046013086015247No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG17770.35545474002996463No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG16870.3374519676030108No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15930.3186490719570814No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC15510.3102477781578363No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13930.27864291100829525No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG13820.276442572156112No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG13760.27524238732764844No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12510.2502385367346571No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12470.2494384135156814No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11760.23523622637886235No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11750.23503619557411842No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11550.2310355794792398No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG11270.22543471694640976No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC10970.2194337928040918No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10170.2034313284245774No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10010.2002308355486745No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9270.18542855599762365No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT9160.1832282171454404No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8610.17222652288452422No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC7640.15282353482436295No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7600.15202341160538724No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7560.1512232883864115No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7500.15002310355794793No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7490.149823072753204No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7170.14342208700139822No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG7010.1402215941254953No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6950.13902140929703174No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6930.13862134768754386No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG6650.13302048515471385No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG6570.1314202387167624No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG6560.13122020791201844No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG6350.12701956101239592No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6240.12481922216021268No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6210.12421912974598089No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6120.1224188525032855No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5780.11561780514199185No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5460.10921681939018608No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5050.10101555639568494No Hit
GGTTTCCGTTTCGAAGTTAAGAAACGTGACG5010.10021543317670922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)