FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007927025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541210
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA28420.5251196393266938No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT26270.485393839729495No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA24850.45915633487925206No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT21520.39762753829382314No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG20550.3797047356848543No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC20100.3713900334435801No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG18730.3460763843979232No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG18670.34496775743242No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT16760.309676465697234No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA15260.28196079155965337No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG14420.2664400140426082No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG13890.25664714251399645No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG13670.2525821769738179No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA13200.24389793241070937No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG12480.23059440882467064No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG12170.22486650283623733No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12080.22320356238798247No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT11090.20491121745717927No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG11090.20491121745717927No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG10970.20269396352617283No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT10420.1925315496757266No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA10140.1873579571700449No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG9980.18440161859536963No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA9740.17996711073335675No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG9740.17996711073335675No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8530.15760980026237506No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8420.1555773174922858No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG8320.15372960588311377No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG8120.15003418266476967No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG7850.14504536132000517No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7730.14282810738899873No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA7330.13543726095231057No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7290.13469817630864175No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG7190.1328504646994697No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA7000.12933981264204283No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT6960.12860072799837402No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG6940.1282311856765396No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6820.12601393174553316No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG6770.12509007594094715No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG6640.12268805084902348No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6550.12102511040076865No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG6520.12047079691801703No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6480.11973171227434823No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6230.11511243325141812No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC6090.11252563699857725No Hit
TGCCCCATTTAAATCAGCCTGTCACTGGACAACGTGACGGGTCT5930.109569298423902No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG5520.10199368082629663No Hit
CAGAGAGGCGAAGCTCAGAAACTGACCCGCGTTAGCTTCGGTAG5500.10162413850446222No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5450.10070028269987621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)