FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007927026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541210
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT66331.2255871103638145No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC28310.5230871565566046No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT25860.4778182221318897No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC22620.4179523659947155No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG22230.4107462907189446No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG21780.40243158847767035No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG20210.3734225162136694No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG18760.3466306978806748No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17890.33055560688087804No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC17590.3250124720533619No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA17250.31873025258217696No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16290.3009922211341254No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG15560.2875039263871695No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG14930.2758633432493856No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG14090.2603425657323405No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14010.25886439644500286No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13680.25276694813473516No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13450.24851721143363945No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC12840.23724617061768996No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG12460.23022486650283625No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC12300.22726852792816096No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG12110.2237578758707341No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10810.19973762495149758No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10500.19400971896306424No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT10210.18865135529646532No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9280.17146763733116535No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC9070.16758744295190406No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8900.16444633321631158No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8500.15705548677962344No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG8420.1555773174922858No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8090.14947986918201805No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7930.14652353060734283No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7890.145784445963674No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7730.14282810738899873No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG7400.136730659078731No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG7360.13599157443506216No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG7320.13525248979139337No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG7280.13451340514772453No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7070.13063321076846326No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6900.12749210103287079No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6800.12564438942369874No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6730.12435099129727832No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6640.12268805084902348No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6330.11696014486059014No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG5910.10919975610206759No Hit
GGTTTCCGTTTCGAAGTTAAGAAACGTGACG5670.10476524824005469No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)