FastQCFastQC Report
Wed 31 May 2023
EGAF00007927028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516245
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78281.516334298637275No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT60831.1783164970120776No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC26510.5135158694030935No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT23340.45211091632848743No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG20820.4032968842313243No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC20750.40194093889529203No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20430.3957423316448585No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG18660.3614562852908987No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG18410.3566136233764976No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC16230.31438561148291994No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA16130.3124485467171595No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG15150.29346531201270715No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA14660.28397369466048095No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG14360.2781625003631996No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13060.2529806584083139No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12980.25143100659570555No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12660.24523239934527213No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12620.24445757343896793No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC11770.22799252293000416No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11610.22489321930478745No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG11430.22140650272641865No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10860.21036523356158413No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10140.19641836724810896No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT9520.1844085657003942No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9220.17859737140311285No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8250.15980784317523655No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC8190.15864560431578031No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG7960.15419035535453127No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7750.15012251934643434No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7740.14992881286985832No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7710.14934769344013016No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7430.14392391209600092No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7420.14373020561942487No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7370.14276167323654465No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG7000.135594533603231No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG7000.135594533603231No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6650.12881480692306949No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG6630.12842739396991737No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6460.12513438386812462No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG6400.12397214500866836No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6320.12242249319606002No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6230.12067913490687561No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6100.11816095071138703No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5450.10557002973394417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)