FastQCFastQC Report
Wed 31 May 2023
EGAF00007927093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454956
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT56291.2372625045059302No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC45320.9961402860936003No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA31670.6961112722988596No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA31560.6936934560704772No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA29980.6589648229718917No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT25980.5710442328488908No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC23120.5081810109109453No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT22800.5011473637011051No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT22330.49081669436165254No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA20780.45674746568898966No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT20520.4510326273309946No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT16220.3565179929487687No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT15600.3428903014797035No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT13400.294533976912053No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG10450.22969254169633987No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT10440.22947274022103234No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG9920.21804306350504227No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA9910.21782326202973473No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG9780.2149658428507372No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG9490.20859160006681965No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA8870.1949639085977545No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC8150.17913820237561434No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA7840.17232435664108178No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT7530.16551051090654922No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG7210.15847686369670913No Hit
GCCCTGACCGCGTGGGCCGGTGGACGGACCCTCACCCGGGTCGT7070.1553996430424041No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA7040.1547402386164816No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC6830.15012440763502405No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6730.14792639288194903No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC6590.14484917222764399No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6520.14331056190049146No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6500.14287095894987648No Hit
CACGCAGGCACCACGACGGCGACGAGACGGCGGCGGAGTAGGTT5340.11737398781420622No Hit
CTGTTCGGTTTCCAGTTCGCCTACGCTCACTTTGGTGTTGTTAC5330.11715418633889872No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG5310.1167145833882837No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA5030.11056014207967363No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4960.10902153175252113No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA4860.1068235169994461No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG4800.10550470814760107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)