FastQCFastQC Report
Sun 21 May 2023
EGAF00007927096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457485
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG59071.2911898750778714No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC57271.251844322764681No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT36350.7945615703247101No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG36320.7939058111194903No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA34840.761555023661978No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG28630.6258128681814704No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26880.5875602478769796No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC26490.5790353782091217No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG23540.514552389695837No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG23110.5051531744210193No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT23080.5044974152157994No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC21060.46034296206433No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG20090.439140081095555No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT19630.4290851066155174No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC17670.38624217187448767No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT15480.3383717498934391No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14850.3246008065838224No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC12000.2623036820879373No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC11700.25574609003573884No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC10720.23432462266522402No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA10460.22864137621998537No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT9220.20153666240423185No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC9120.19935079838683234No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC8920.1949790703520334No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC8430.18426833666677597No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7740.16918587494671955No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7710.16853011574149973No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA6960.15213613561100364No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6950.15191754920926367No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6920.15126179000404383No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG6840.14951309879012428No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6780.1482015803796846No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6200.13552356907876761No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6060.13246335945440835No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5530.1208782801621911No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5240.11453927451173262No Hit
GACAAAGCGGAAGCTCAGAGCACCCCGGAAC5160.11279058329781305No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5030.10994896007519372No Hit
CGTCCTTCCGATATCGACACCGCGGCACGCG4940.1079816824595342No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC4750.10382854082647518No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC4690.1025170224160355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)