FastQCFastQC Report
Wed 31 May 2023
EGAF00007927169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457792
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT26080.5696910387250105No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC23380.5107122885502586No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT18610.40651649657486366No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC18090.3951576261708374No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC17890.3907888298615965No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT14050.30690794072417166No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12990.283753320285195No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG12510.2732682091430169No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC12260.2678072137564658No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG12250.2675887739410038No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA12030.2627830980008388No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT11870.25928806095344614No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA11270.2461816720257235No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10900.23809939885362785No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG10690.23351216272892492No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG9380.20489654690339718No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG9120.19921711170138406No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA9010.19681427373130153No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT8770.1915717181602125No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8530.18632916258912344No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8400.18348944498811687No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT8240.1799944079407242No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA8100.17693625052425555No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA8040.1756256116314833No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT7960.17387809310778693No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT7850.17147525513770445No Hit
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG7800.17038305606039422No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT7320.15989794491821613No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG6810.1487575143296519No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT6420.14023836152663216No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6340.13849084300293582No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG5910.12909793093806796No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5860.12800573186075773No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG5730.12516601425975116No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG5650.1234184957360548No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT5590.12210785684328253No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG5520.12057877813504825No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5450.11904969942681393No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG5290.11555466237942122No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG5190.11337026422480079No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG4940.10790926883824968No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC4940.10790926883824968No Hit
TTCCATCGGCTGCTGCGGGCTTACCTGGGTCAGCTGCGGTGGCT4880.10659862994547742No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG4580.10004543548161611No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)