FastQCFastQC Report
Sun 21 May 2023
EGAF00007927170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457792
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA55001.2014189850412413No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA27390.5983066545505382No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA24010.5244739969243674No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC23430.5118044876275689No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT19730.43098175590661264No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19240.42027820494897244No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14000.3058157416468614No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13310.2907433943799804No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA13170.28768523696351184No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT12910.28200580176149864No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12400.27086537117293447No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT12040.26300153781630087No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11820.2581958618761359No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10900.23809939885362785No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10640.23241996365161469No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10360.22630364881867748No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10240.22368237103313296No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9090.1985617922549979No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9030.19725115336222562No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8980.19615895428491542No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8480.18523696351181324No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA8270.1806497273871103No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8060.1760624912624074No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA7840.1712568153222424No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC7710.16841709772123584No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG7700.16819865790577382No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT7240.1581504263945198No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7220.1577135467635957No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6860.14984971340696213No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6620.14460715783587305No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC6370.13914616244932196No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC6350.13870928281839787No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6220.1358695652173913No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6220.1358695652173913No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5880.12844261149168182No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5760.1258213337061373No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5700.12451069481336503No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5570.12167097721235845No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5530.12079721795051027No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5400.11795750034950371No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5250.11468090311757305No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5230.11424402348664896No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5000.10921990773102196No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC4980.10878302810009786No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC4940.10790926883824968No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC4710.10288515308262268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)