FastQCFastQC Report
Wed 31 May 2023
EGAF00007927172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439352
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA51711.1769606147235019No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA25700.5849523844206923No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA23090.5255467142518983No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22290.507338079717402No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC22020.5011926655620096No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT17830.40582494218758536No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG17750.4040040787341357No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13770.31341612192501683No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12740.2899725049618529No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12180.2772264607877055No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT12110.2756332052659371No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT12030.2738123418124875No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11240.2558313152096724No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11090.2524171962344544No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10660.24263005517216266No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10450.23785028860685736No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9690.22055208579908592No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9260.2107649447367942No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8970.2041643147180393No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8860.20166062746954605No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8370.1905078388171671No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8090.1841348167300934No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7900.17981026602815053No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA7660.17434767566780168No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC7540.17161638048762723No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT7450.16956790910249642No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG7270.16547096633223474No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC7000.15932555217684224No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA6980.15887033631347985No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6730.15318013802144975No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6620.15067645077295652No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6310.14362060489083922No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6100.13884083832553396No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6070.13815801453049037No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC6070.13815801453049037No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5870.1336058558968663No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5440.12381871483457456No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5290.12040459585935652No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5250.11949416413263168No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5040.11471439756732642No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5020.11425918170396403No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA4860.11061745479706478No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC4730.10765855168520912No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT4520.10287878511990385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)