FastQCFastQC Report
Wed 31 May 2023
EGAF00007927174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448808
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA53631.1949430491435091No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA26550.5915669952407265No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA22960.5115773337373665No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC22820.5084579597511631No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19940.4442879806064063No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT19620.4371579829236556No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG18590.41420830288230154No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13100.291884280137609No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13050.29077021799967917No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT12780.2847542824548582No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12150.27071709951694267No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT12130.27027147466177076No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11750.26180460241350423No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11730.26135897755833226No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10820.24108304664800986No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10560.23528992353077485No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10040.22370367729630486No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9960.2219211778756172No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9150.20387337124115434No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8830.19674337355840357No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8550.19050462558599668No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8530.19005900073082477No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA8100.18047806634462843No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8040.17914119177911267No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC8030.1789183793515267No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA7460.16621807097912691No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA7220.16087057271706387No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG7030.1566371365929306No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT6890.15351776260672717No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC6800.15151245075845352No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6630.14772463948949216No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6480.14438245307570274No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6100.13591558082743624No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6080.1354699559722643No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5950.13257339441364682No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5880.13101370742054508No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC5860.13056808256537317No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5800.1292312079998574No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5790.12900839557227142No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5420.12076433575159087No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5240.11675371205504359No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5070.11296590078608225No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC4990.11118340136539455No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC4730.10539027824815958No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4730.10539027824815958No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT4680.10427621611022976No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC4670.1040534036826438No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG4530.10093402969644034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)