FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007927301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540831
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA27890.5156878951095628No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA24090.4454256505266895No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT21780.40271360184604804No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC20420.3775671143111249No Hit
CCAGGCAGAACCGTGGTGGTTACGGGATGCGCTACCAGAATGGC20360.37645771044929005No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA14540.2688455358513103No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12230.22613348717066883No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT12010.22206567301060776No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC11560.2137451440468464No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA11290.20875282666858963No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT10840.20043229770482832No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT9470.17510090952626606No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG9210.1702934927916484No Hit
GGTACGCTCGACGTGGCAACGTGCCAGCAGCAGACGCAGACCTT8990.1662256786315873No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC8420.1556863419441563No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG7590.14033958852210765No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7550.1395999859475511No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT7480.1383056814420771No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG7400.13682647629296396No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG7350.13590197307476828No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA7180.13275866213290288No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7100.13127945698378976No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT6990.12924554990375922No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG6570.12147972287091531No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC6280.11611760420538024No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC6270.11593270356174111No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6110.11297429326351485No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5850.1081668765288972No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA5660.10465376429975354No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG5600.10354436043791869No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT5500.10169535400152728No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC5480.101325552714249No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG5450.10077085078333158No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)