FastQCFastQC Report
Sun 21 May 2023
EGAF00007927304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515016
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT54861.0652096245553535No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44250.8591966074840394No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC26210.5089162278453485No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21620.41979278313683455No Hit
CGTTCTCGCCACGAGAACACCTCTCAGGTTC20220.39260916165711357No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15360.2982431613775106No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13560.2632927909035836No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13030.2530018484862606No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12610.24484676204234432No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC12400.24076921882038615No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11550.2242648772076984No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG9260.17980023921586902No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG9130.17727604579275208No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT8880.17242182767137332No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8040.1561116547835407No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG8020.1557233173338304No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC8010.15552914860897527No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7690.14931574941361045No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7600.14756823088991408No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC7250.14077232551998387No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7220.1401898193454184No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7070.13727728847259113No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC6900.1339764201500536No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6780.1316463954517918No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA6620.12853969585410938No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6610.12834552712925423No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6600.1281513584043991No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6560.1273746835049785No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC6550.12718051478012332No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6360.12349130900787549No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6000.11650123491309008No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC5630.10931699209344953No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5460.10601612377091198No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5250.10193858054895381No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC5190.10077356819982292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)