FastQCFastQC Report
Sun 21 May 2023
EGAF00007927359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505180
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA19640.38877231877746543No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA17650.34938041886060417No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT16410.3248347123797458No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC14020.27752484263034954No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT13320.2636683954234134No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC12790.25317708539530465No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT12660.25060374519973083No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA10660.2110138960370561No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC10320.2042836216794014No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA10140.20072053525476066No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT9580.18963537748921178No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT9250.18310305237737046No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA8740.1730076408408884No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT8620.1706322498911279No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7860.15558810720931154No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7780.15400451324280454No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG7210.14272140623144225No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA6890.13638703036541433No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG6740.13341779167821372No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6200.12272853240429153No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC6160.12193673542103806No Hit
ACGACGGGAGGAGCGAGAGTTCACAGACGGACGACGACCTGGGC6140.1215408369294113No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5850.11580030880082347No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5570.11025772991804901No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5550.10986183142642227No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT5480.10847618670572864No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5180.10253770933132744No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5110.10115206461063382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)