FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007927362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498773
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA33850.678665445002035No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT30370.6088942264316632No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC18160.364093485413204No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG17300.3468511727779972No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG16760.33602460437914644No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA16310.3270024640467708No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA15680.3143714675814449No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14040.28149077837012026No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG13960.2798868423110313No Hit
CTGAAGGCGACCACCACCCACCTGTTCGAAG12130.2431968049593703No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG12070.24199385291505354No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11870.237984012767331No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11160.22374908024291612No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA8250.16540590609355357No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA7800.15638376576117793No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG7600.15237392561345542No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7430.14896556148789128No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT7400.1483640854657329No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC6450.129317344764051No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5680.1138794601953193No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG5450.10926814402543843No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5130.10285239978908242No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)