FastQCFastQC Report
Wed 31 May 2023
EGAF00007927472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452177
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA46641.0314544968010315No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36530.8078694847371716No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC24300.5374001773641737No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA24050.5318713689550774No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA21840.48299670261866484No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21720.4803428745822985No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13740.30386331016394025No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13470.29789219708211606No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12540.2773250298002773No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12510.27666157279118575No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11790.26073860457298803No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10870.24039258962751311No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG10760.23795991392751067No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10600.23442147654568896No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG10290.2275657541184094No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9770.2160658326274888No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9740.21540237561839723No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9570.21164278590021166No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9200.20346014945474894No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG8390.18554681020927644No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC8060.17824878310926914No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG8060.17824878310926914No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7910.17493149806381128No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG6970.15414317844560868No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT6880.15215280741833398No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6700.14817206536378455No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6660.1472874560183291No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA6630.14662399900923753No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6550.1448547803183267No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6480.14330671396377967No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6130.13556638219104467No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6020.13313370649104223No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6000.1326914018183145No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5850.12937411677285665No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5750.12716259340921807No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5720.1264991364001265No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5540.12251839434557707No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5160.11411460556375048No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4880.10792234014556246No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA4650.10283583640919375No Hit
CCGGAAACTCTGGAACAGCTGACCCTGGTTG4610.10195122706373831No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC4580.10128777005464673No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG4550.10062431304555518No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)