FastQCFastQC Report
Wed 31 May 2023
EGAF00007927474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481681
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA49901.0359553314330439No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA27040.56136737799498No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC26820.5568000398604055No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA24370.5059365015435526No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG22430.46566088344775897No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG15660.3251114326701697No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG14660.30435080478573995No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13990.29044118410317205No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13040.27071858761296375No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13030.27051098133411944No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12540.2603382736707489No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11760.24414498392089373No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11660.2420689211324507No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG11190.23231142602676874No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG11150.2314810009113916No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10730.22276153719993108No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9970.20698346000776446No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG9730.20200090931550133No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9270.19245102048866367No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC8760.1818631002676045No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG8760.1818631002676045No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8660.17978703747916153No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8520.17688054957534136No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG8030.1667078419119708No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT7260.15072215844095987No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7150.14843848937367263No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG7120.14781567053713973No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7080.14698524542176253No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7030.14594721402754104No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA6950.14428636379678667No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6650.13805817543145774No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC6630.13764296287376915No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6500.13494408124879329No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6410.1330756247391946No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6090.12643222381617708No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6080.1262246175373328No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6050.1256017987007999No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5930.12311052335466835No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5850.12144967312391396No Hit
CCGGAAACTCTGGAACAGCTGACCCTGGTTG5500.11418345336436356No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5070.10525638337405877No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG5050.10484117081637016No Hit
CGTCGCCAGCGTGCAATCGACAAACGCCAGC4990.10359553314330439No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC4880.10131186407601712No Hit
CTGATGCGTATGAAGGAAGTATCTAGCGTTT4830.10027383268179564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)