FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007927476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454117
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA48361.0649237971712135No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37260.8204933970760839No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA24640.5425914466976572No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC24020.5289385775031544No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA22940.5051561601966013No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20940.4611146466659473No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13900.30608851903804524No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13150.28957295146405No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13090.2882517060581304No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12270.27019468551056225No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT11990.2640288736162707No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11960.2633682509133109No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG10990.2420081168509437No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10420.2294562854947073No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG10380.22857545522409423No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10070.22174902062684285No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9670.21294071792071206No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9230.20325158494396817No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8980.19774639575263642No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC8280.18233186601690754No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG8270.18211165844925428No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8000.176166054122616No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7890.17374377087843001No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG6890.15172301411310302No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT6480.14269450383931895No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA6470.14247429627166566No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6260.137849937350947No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6260.137849937350947No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6180.13608827680972083No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6130.13498723897145448No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6010.13234474815961525No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC5870.12926184221246947No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5860.1290416346448162No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5800.1277203892388966No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5780.12727997410359004No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5760.1268395589682835No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5650.12441727572409754No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5580.12287582275052464No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5340.11759084112684616No Hit
CCGGAAACTCTGGAACAGCTGACCCTGGTTG5240.11538876545031346No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG4710.10371776436469016No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG4680.10305714166173034No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA4630.10195610382346401No Hit
CTGATGCGTATGAAGGAAGTATCTAGCGTTT4610.10151568868815745No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG4580.10085506598519764No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)