FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007927492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455664
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA59881.314126198251343No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36970.8113434460479652No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC33790.7415551810105692No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA33210.7288265037395976No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA30930.6787896344675024No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19460.42706906843639175No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16970.372423540152393No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15900.34894132518697984No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG14630.321069911162611No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14420.31646125215070753No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12530.274983321043576No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC10950.24030864847782574No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC10680.23438322974823553No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10180.22341023210084623No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC9180.20146423680606762No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8860.19444151831173848No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG8610.18895501948804383No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8590.18851609958214824No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8090.17754310193475895No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8010.17578742231117664No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA7990.1753485024052811No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7880.17293444292285545No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7700.1689841637697953No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT7530.1652533445696829No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6590.14462410899259104No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6580.14440464903964326No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT6380.14001544998068752No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6370.13979599002773974No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6350.13935707012184417No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6290.13804031040415746No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC5750.126189472944977No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5530.1213613539801257No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG5300.11631377506232662No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5230.11477755539169213No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA5040.11060781628568418No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA4650.10204887812072053No Hit
CGCCTGGACGCAGAACAGCGCGCCCAGCAGA4590.10073211840303381No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA4590.10073211840303381No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)