FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007927535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452720
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA18380.405990457678035No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA17110.3779377981975614No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC16100.3556282028626966No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA15760.3481180420569005No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG15130.3342021558579254No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13280.29333804559109383No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG13230.29223361017847677No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG13170.29090828768333626No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG13030.2878158685280085No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA11890.2626347411203393No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG11580.25578724156211347No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG10900.2407669199505213No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT9720.21470224421275844No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9610.2122724863050009No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC9560.21116805089238383No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG9500.20984272839724333No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9460.20895918006714967No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT8840.195264180950698No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA8190.18090652058667608No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8120.17936031100901217No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG7600.16787418271779467No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG7490.1654444248100371No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT7150.15793426400424104No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG7080.15638805442657713No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG6840.1510867644460152No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC6840.1510867644460152No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT6580.14534370030040644No Hit
GAACACTACAACTTTTTTGTTGCCGATCAGATAAATACCGGCCG6400.141367732814985No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG6360.14048418448489133No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6350.1402632974023679No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC6300.13915886198975083No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT6200.1369499911645167No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6170.13628732991694645No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6050.1336366849266655No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5860.12943983035872061No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC5480.12104612122283089No Hit
GGCCGGCTTTTTCGGTGGCTGAACTGGCGGTTTCGGAGCACCCG5440.12016257289273723No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG5240.11574483124226895No Hit
ACCGCCCAGAGAATCAGCGATCAGATGGGAACGGTCGAACAGGT5210.11508216999469871No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC5110.11287329916946458No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5040.11132708959180067No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG4980.1100017670966602No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG4840.10690934794133239No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG4840.10690934794133239No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA4770.1053631383636685No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG4720.10425870295105143No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA4580.10116628379572364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)