FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007927536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452720
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT45631.0079077575543383No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC23970.5294663368086234No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA20520.4532602933380456No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG17140.37860045944513165No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA16700.36888142781410144No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG16100.3556282028626966No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG15060.3326559462802615No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG14350.31697296342109915No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14340.3167520763385757No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13720.30305707722212405No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13040.2880367556105319No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11980.26462272486305No Hit
TCCTACACCAACGGCAAACTGACTATCACCC11110.245405548683513No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10710.23657006538257644No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA10500.23193143664958474No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG9890.21845732461565648No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9890.21845732461565648No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9770.2158066796253755No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC9740.21514401837780528No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA9040.1996819226011663No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8730.19283442304294046No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8690.1919508747128468No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8580.18952111680508923No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG8150.18002297225658245No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7900.17450079519349707No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7340.1621311185721859No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG7310.16146845732461565No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7140.1577133769217176No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6900.15241208694115568No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6640.14666902279554692No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6600.14578547446545326No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6580.14534370030040644No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG6520.14401837780526594No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6470.14291394239264887No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6300.13915886198975083No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6300.13915886198975083No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA6280.13871708782470402No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6200.1369499911645167No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6060.1338575720091889No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA5940.13120692701890793No Hit
GTAGGCTCCGTGTTCAAAGAAATCATTAACC5830.1287771691111504No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG5250.11596571832479237No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5180.11441950874712847No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5000.11044354126170702No Hit
GCGAGCCAGGCCAAAAAAGTAGTTCAGCAGC5000.11044354126170702No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4950.10933910584908994No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4890.10801378335394947No Hit
AGCCTGGACCAGATTCCGGGCTATCTGAACC4610.10182894504329387No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4550.10050362254815338No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC4530.10006184838310656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)