FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007927538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429278
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT43311.0089033213908003No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36680.8544579503258961No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC22810.5313573022610057No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA19280.44912620725963126No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG16750.39019004002068586No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15110.35198635849030235No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG14140.3293902785607462No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13990.3258960393963818No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG13910.3240324451753875No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13530.3151803726256645No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13300.3098225392403058No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12650.29468083619472696No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10860.25298291549997903No Hit
TCCTACACCAACGGCAAACTGACTATCACCC9840.22922208918230144No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT9820.22875619062705288No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG9420.21943821952208126No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG9260.2157110310800926No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA9140.21291563974860112No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC9120.21244974119335255No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9000.20965434986186104No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA8890.20709190780799389No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7960.1854276249889349No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7890.18379698004556488No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG7470.1740131103853447No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG7110.16562693639087026No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7080.1649280885579974No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7020.16353039289225163No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6650.1549112696201529No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6420.14955343623479425No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6360.1481557405690485No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG6180.14396265357181126No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6110.14233200862844125No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6050.14093431296269548No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5960.1388377694640769No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5790.13487763174446396No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5780.13464468246683967No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5680.13231518969059677No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5630.13115044330247533No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA5580.12998569691435388No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5290.12323016786324945No Hit
GTAGGCTCCGTGTTCAAAGAAATCATTAACC5260.12253132003037658No Hit
GCGAGCCAGGCCAAAAAAGTAGTTCAGCAGC4840.1127474503701564No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4840.1127474503701564No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC4770.11111680542678637No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG4550.10599192131905198No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC4480.10436127637568197No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT4480.10436127637568197No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4460.10389537782043338No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT4450.1036624285428091No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)