FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007927545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520816
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC39420.756889189272219No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT33420.641685355288624No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA32540.6247887929710301No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA30200.5798592977174281No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG28600.5491382753218027No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT28040.5383859174833339No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA25410.48788823692052474No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG23950.45985530398451663No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT22920.44007864581733275No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG22880.43931062025744216No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC21360.41012564898159815No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG20370.39111701637430496No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG20280.389388958864551No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA20030.3845887991152346No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT19510.3746044668366563No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA19410.3726844029369297No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG17870.3431154188811404No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG17850.34273140610119507No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT16820.32295474793401124No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA16620.31911462013455805No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT14730.28282541242972564No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG14590.28013732297010846No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT14470.27783324629043654No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA13750.26400878621240514No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG13680.26266474148259655No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG13470.2586326072931707No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC13010.24980031335442845No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT13010.24980031335442845No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT12500.24000798746582286No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC12250.2352078277165064No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT12230.2348238149365611No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA11670.22407145709809223No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA11620.2231114251482289No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC11470.22023132929863903No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA10920.20967097785014288No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA10690.20525483088077173No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC10580.20314276059107245No Hit
GGTACGCTCGACGTGGCAACGTGCCAGCAGCAGACGCAGACCTT9970.19143037080274034No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT9120.17510982765506436No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA8320.1597493164572517No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8190.15725323338760713No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG8150.15648520782771652No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG7990.153413105588154No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG7950.15264508002826335No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG7790.14957297778870082No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7780.14938097139872814No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG7700.14784492027894688No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC7680.14746090749900156No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA7610.14611686276919295No Hit
CACATCGTCACGGAACTGTTTGGTCGGCATGGACAGGGTCAGAG7360.1413167030198765No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG7170.137668581610396No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG7010.13459647937083347No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6940.13325243464102485No Hit
CAGTTCGGTCAGCATCGGCAGGTCAACCGCGATGCCCGCAGATT6840.13133237074129828No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6800.13056434518140764No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT6250.12000399373291143No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT6190.11885195539307548No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG6030.11577985315351295No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA5520.10598752726490737No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5520.10598752726490737No Hit
CCAGTTACCATCTTCGGTGGTGCGTTCGACGTGGCAACGTGCCA5500.10560351448496207No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG5490.10541150809498939No Hit
CAGCGCGCGCAGGCCCTCAGCGATGTTTTCGAACTTCGGGTTGG5440.10445147614512611No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG5330.10233940585542686No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA5320.1021473994654542No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT5220.10022733556572763No Hit
CAGTGCACGCAGGCCTTCAGCGATGTTTTCGAACTTTGGGTTGG5220.10022733556572763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)