FastQCFastQC Report
Wed 31 May 2023
EGAF00007927546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520816
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC57651.106916838192375No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT45290.8695969401861694No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG36020.6916070166815151No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG34990.6718303585143314No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA34220.6570458664864367No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG31580.6063561795336548No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT29280.5621947098399435No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT28750.5520183711713926No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG27880.5353138152437713No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC26300.5049768056280912No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG25820.49576049890940366No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC25020.480399987711591No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC24400.46849559153328624No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG23840.45774323369481734No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG23320.44775890141623914No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG22400.43009431353875455No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG20790.39918128475315656No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG17580.3375472335719333No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA17160.3294829651930816No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC16820.32295474793401124No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG16680.320266658474394No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC15880.30490614727658133No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15410.2958818469478664No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG14190.2724570673712021No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT13860.2661208565021044No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG13770.26439279899235046No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT13620.2615127031427606No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC13580.26074467758286995No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT13560.26036066480292464No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT12700.24384811526527603No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC12640.24269607692544007No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG12400.2380879235660963No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC12350.23712789161623296No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG11680.2242634634880649No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC11540.22157537402844765No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT11180.21466314398943198No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG10530.20218272864120918No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC9890.18989431968295906No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9460.18163804491413474No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC9350.17952597462443548No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9160.175877853214955No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA8970.1722297318054745No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC8600.16512549537648613No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8460.16243740591686892No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT8460.16243740591686892No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG8400.16128536757703296No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8340.160133329237197No Hit
GAACGTAACGACCTGGATGTTCAGGGTATCA8090.15533316948788056No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC8050.15456514392798992No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC8010.15379711836809928No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG7640.14669288193911093No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7390.14189272218979448No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC7320.14054867745998587No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7160.13747657522042334No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7140.13709256244047802No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG7010.13459647937083347No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6830.13114036435132562No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6820.13094835796135296No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6770.12998832601148966No Hit
CGTGCTCGTGCAGTTATTGACCTGGCAGACG6540.12557217904211854No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG6100.11712389788332156No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5990.11501182759362232No Hit
GAACAGGGTCCGGCAGACGATCCGGGTGAAG5820.11174771896408714No Hit
GAACAGGGCCCGGCGGACGATCCGGGTGAGG5800.1113637061841418No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5700.10944364228441524No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC5680.1090596295044699No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5600.10752357838468864No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5470.10502749531504409No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT5440.10445147614512611No Hit
GACGACCCGGGTGAAGGCCCGTCTACTGGCC5380.10329943780529016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)