FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007927547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494934
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC37240.7524235554639608No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT31820.6429140046955756No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA30860.6235174790982232No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA28240.5705811279887824No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT27150.5485579895501218No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG26860.5426986224425884No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA23230.4693555100275996No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG21850.4414730044814056No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT20840.42106624317585784No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG20790.4200560074676623No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG19950.403084047569979No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA19910.40227585900342266No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC19670.39742672760408454No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG19530.39459806762113736No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA18270.36914012777461236No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT17850.3606541478257707No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG17720.3580275349844626No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG16950.34246990507825287No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA16790.33923715081202743No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT14930.3016563824671572No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT13440.27155135836293326No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG13430.2713493112212942No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG13140.2654899441137606No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT12850.2596305770062271No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG12560.25377120989869356No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA12560.25377120989869356No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC12460.25175073848230267No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT12110.24467908852493464No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT11300.22831327005216856No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT10860.21942319582004874No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA10800.2182109129702142No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC10790.21800886582857512No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA10630.21477611156234971No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA10570.21356382871251522No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC10540.21295768728759795No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC10260.2073003673217035No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA10110.20426966019711718No Hit
GGTACGCTCGACGTGGCAACGTGCCAGCAGCAGACGCAGACCTT9290.18770179458271202No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT8820.17820557892567493No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA7980.16123361902799163No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT7950.16062747760307436No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG7760.15678858191193168No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7620.15395992192898447No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7500.1515353562293154No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA7490.15133330908767634No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG7350.14850464910472913No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG7150.14446370627194735No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG7130.14405961198866918No Hit
CACATCGTCACGGAACTGTTTGGTCGGCATGGACAGGGTCAGAG7070.1428473291388347No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG7050.14244323485555652No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC7020.14183709343063924No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6950.14042276343916563No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG6420.12971426493229402No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6290.12708765209098585No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT6190.12506718067459502No Hit
CAGTTCGGTCAGCATCGGCAGGTCAACCGCGATGCCCGCAGATT5910.11940986070870055No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5750.11617710644247516No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG5700.11516687073427972No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG5490.1109238807598589No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5280.10668089078543806No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA5270.10647884364379898No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT5130.10365018366085176No Hit
CCAGTTACCATCTTCGGTGGTGCGTTCGACGTGGCAACGTGCCA5120.10344813651921266No Hit
CAGCGCGCGCAGGCCCTCAGCGATGTTTTCGAACTTCGGGTTGG5060.10223585366937814No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA5050.10203380652773905No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG5040.10183175938609995No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG4990.10082152367790453No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)