FastQCFastQC Report
Sun 21 May 2023
EGAF00007927548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494934
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC54141.0938832248340182No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT42820.8651658604985716No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41470.8378894963772948No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG33500.6768579244909422No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG32770.6621084831512888No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA32150.6495815603696654No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG29720.6004841049513672No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT27100.5475477538419264No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT26600.537445396759972No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG24480.49461140273248555No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG24230.4895602241915083No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC23830.4814783385259449No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG23080.4663248029030133No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC22830.46127362436203617No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC22090.44632213588074365No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG21770.43985662734829295No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG20950.42328876173388774No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG20080.4057106604112871No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG16480.33297368942121575No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG15970.32266928519762234No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC15560.3143853523904197No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA15150.3061014195832172No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC15130.305697325299939No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13880.28044143259505305No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT13600.27478411262915864No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG13010.2628633312724525No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT12800.2586203412980317No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC12750.2576101055898362No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC12190.24629546565804733No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT12180.24609341851640826No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG11810.238617674275762No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG11660.23558696715117572No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC11630.23498082572625845No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG11230.22689894006069494No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT11040.22306004436955232No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT10970.2216457143780787No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC10520.21255359300431978No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG9950.20103690593089182No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8770.1771953432174795No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8750.17679124893420134No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC8720.17618510750928407No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA8580.17335644752633686No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC8260.16689093899388607No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8020.16204180759454795No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT8020.16204180759454795No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7810.15779881762012712No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC7660.15476811049554082No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG7590.1533537805040672No Hit
GAACGTAACGACCTGGATGTTCAGGGTATCA7560.15274763907914995No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7100.14345347056375193No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7000.14143299914736107No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC7000.14143299914736107No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG6960.14062481058080473No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC6710.13557363203982753No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6620.13375520776507574No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG6350.1282999349408204No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6310.12749174637426405No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6280.12688560494934678No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6180.12486513353295592No Hit
CGTGCTCGTGCAGTTATTGACCTGGCAGACG5730.11577301215919698No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5660.11435868216772337No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5650.11415663502608428No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5490.1109238807598589No Hit
GAACAGGGCCCGGCGGACGATCCGGGTGAGG5430.10971159791002437No Hit
GAACAGGGTCCGGCAGACGATCCGGGTGAAG5380.10870136220182892No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5330.1076911264936335No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT5330.1076911264936335No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5160.10425632508576901No Hit
GACGACCCGGGTGAAGGCCCGTCTACTGGCC5140.10385223080249084No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5000.1010235708195436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)