FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007927549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498554
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC38190.7660153162947244No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA31770.6372429064855563No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT31360.629019123304597No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT28260.5668392992534409No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG27960.5608218969259098No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA27930.5602201566931566No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA22960.4605318581337227No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG22910.4595289577458008No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG21560.4324506472719104No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT20980.4208170027720167No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG20420.4095845184272917No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC20290.4069769774186949No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA20040.40196247547908553No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG19370.38852361028093246No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA18180.36465458104839193No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT18030.3616458798846263No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA17370.34840759476405764No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG17370.34840759476405764No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG16610.33316350886764523No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT16090.3227333448332578No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT14550.2918440128852642No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT14420.2892364718766673No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG13930.279408048075033No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG13120.2631610617906987No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG13030.2613558410924393No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC12660.2539343782218175No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA12550.25172799736838936No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA12120.2431030540322613No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT11920.23909145248057379No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC11790.23648391147197695No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT11530.23126882945478322No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT11530.23126882945478322No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA11430.22926302867893952No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA10690.2144201029376958No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC10450.20960618107567086No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC10180.20419051898089274No Hit
GGTACGCTCGACGTGGCAACGTGCCAGCAGCAGACGCAGACCTT9750.19556557564476465No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA9690.1943620951792584No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT8300.16648146439503042No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8160.1636733433088492No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG8130.16307160307609608No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG8130.16307160307609608No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA7970.1598623218347461No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG7640.15324317927446174No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG7420.1488304175676055No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG7290.14622287655900865No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA7270.14582171640383992No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG7190.14421707578316492No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7060.14160953477456806No Hit
CACATCGTCACGGAACTGTTTGGTCGGCATGGACAGGGTCAGAG6970.13980431407630867No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC6670.13378691174877747No Hit
CAGTTCGGTCAGCATCGGCAGGTCAACCGCGATGCCCGCAGATT6650.13338575159360871No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG6390.128170669576415No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6320.12676660903332437No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG6300.12636544887815565No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT6260.12556312856781812No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5940.11914456608511817No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA5740.11513296453343068No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG5700.11433064422309319No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG5600.11232484344724945No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA5470.10971730243865259No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG5300.10630744111971822No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG5250.10530454073179635No Hit
CAGCGCGCGCAGGCCCTCAGCGATGTTTTCGAACTTCGGGTTGG5150.10329873995595261No Hit
CCAGTTACCATCTTCGGTGGTGCGTTCGACGTGGCAACGTGCCA5010.10049061886977138No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)