FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007927550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498554
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC54741.0979753446968634No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT44770.8979970073452425No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42880.8600873726817957No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG33330.6685333985887186No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA32780.657501494321578No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG32590.6536904728474748No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG30470.6111674963995877No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT28270.5670398793310254No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT26000.5215082017193724No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC25110.5036565748143631No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG24930.5000461334178444No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG23940.4801887057369914No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG23820.4777817448059789No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC22580.45290981518551654No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC22520.45170633472001026No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG22400.4492993737889978No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG21640.4340552878925853No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG19940.3999566747032418No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG17220.345398893600292No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA16330.3275472666952828No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC15580.3125037608764547No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG14990.30066953629897664No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14940.29966663591105475No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT13930.279408048075033No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT13900.27880630784227983No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC13610.272989485592333No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT13290.26657092310963304No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG12880.25834713992867375No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC12790.25654191923041436No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG12560.25192857744597374No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG12060.24189957356675504No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT11640.23347521030821133No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC11600.23267288999787383No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG11380.2282601282910176No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC11370.22805954821343322No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC11360.22785896813584888No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT11120.2230450462738239No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG10340.20739980022224272No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9060.18172555029144286No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC9000.1805220698259366No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8770.17590872804149602No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC8350.16748436478295228No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8330.16708320462778353No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC8260.16567914408469295No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA8180.16407450346401795No Hit
GAACGTAACGACCTGGATGTTCAGGGTATCA7850.1574553609037336No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7810.1566530405933961No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7710.15464723981755238No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC7690.15424607966238363No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG7680.15404549958479924No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG7170.14381591562799617No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7150.14341475547282742No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC7000.1404060543090618No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6860.13759793322288058No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6750.13539155236945247No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6660.13358633167119308No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG6560.13158053089534935No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6440.12917356996433688No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6420.1287724098091681No Hit
CGTGCTCGTGCAGTTATTGACCTGGCAGACG6320.12676660903332437No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG6090.12215326724888376No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5770.1157347047661838No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5710.11453122430067757No Hit
GAACAGGGTCCGGCAGACGATCCGGGTGAAG5500.11031904267140571No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5440.10911556220589948No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5340.10710976143005572No Hit
GAACAGGGCCCGGCGGACGATCCGGGTGAGG5260.10550512080938074No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5200.10430164034387447No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC5170.10369990011112136No Hit
GACGACCCGGGTGAAGGCCCGTCTACTGGCC4990.10008945871460262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)