FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007927624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430952
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT49971.1595258868737122No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC21340.49518275817260393No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG18850.4374037015723329No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18140.420928548887115No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG16140.3745196680836845No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG15930.36964673559932426No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC13880.322077632775808No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12410.2879671053852865No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12120.2812378176687891No Hit
ATCGGCTCCCTGGCTGGTGCAATTGGCGGTA11670.27079581948801723No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11270.2615140433273311No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC11100.25756928845903954No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA10400.24132618017783883No Hit
GACGCCGCGTCCAGCAGCTCTGCAGGTCAGT10260.2380775585215987No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10200.23668529209749578No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9890.22949191557296406No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT9580.2222985390484323No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA9440.21904991739219218No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG9240.21440902931184913No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9210.21371289609979763No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8890.20628747517124876No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG8580.199094098646717No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8410.19514934377842544No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG8160.18934823367799664No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7970.18493939000167073No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7890.1830830347695335No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7490.1738012586088474No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6910.16034268317585254No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6830.1584863279437153No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6670.15477361747944085No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG6650.15430952867140657No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6480.15036477380311497No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6420.14897250737901205No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6260.1452597969147376No Hit
GACGCCGCGTCTTCTTCTTCCGCGGGTCAGT6170.14317139727858325No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5990.13899459800627448No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5640.13087304386567414No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5510.12785646661345115No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG5080.1178785572407136No Hit
GATGCAGCTTCCAACTCTGCAAACCGTCAGG4980.11555811320054206No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG4960.11509402439250774No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4960.11509402439250774No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG4860.11277358035233621No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA4690.10882882548404463No Hit
AAAGAGCTGTACGATTCCCCGTCTGCGCAGC4510.10465202621173589No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC4440.1030277153836158No Hit
AACTATATCGACTCCGTTCTGAACGAGGTTC4400.10209953776754721No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG4350.10093931574746144No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)