FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007927628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433290
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT49611.1449606499111449No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC21060.48604860486048607No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19590.45212213529045214No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG19300.44542915830044544No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG16380.37803780378037805No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG16170.37319116527037316No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC14000.3231092340003231No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG12900.2977220799002977No Hit
ATCGGCTCCCTGGCTGGTGCAATTGGCGGTA12750.29426019525029423No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12650.29195227215029196No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11760.27141175656027144No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC10990.25364074869025366No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG10770.24856331787024857No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA10540.24325509474024323No Hit
GACGCCGCGTCCAGCAGCTCTGCAGGTCAGT10290.23748528699023747No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9870.22779200997022778No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA9760.22525329456022525No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG9700.22386854070022386No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT9400.21694477140021695No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9210.21255971751021255No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG8870.2047127789702047No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8440.1947887096401948No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8420.19432712502019434No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7990.1844030556901844No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7930.183018301830183No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7920.18278750952018277No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7820.18047958642018047No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7260.16755521706016757No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6890.15901590159015902No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6700.15463084770015462No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6530.1507073784301507No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6400.1477070784001477No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG6390.14747628609014748No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6110.141014101410141No Hit
GACGCCGCGTCTTCTTCTTCCGCGGGTCAGT6070.1400909321701401No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC6070.1400909321701401No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG5950.1373214244501373No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG5250.12116596275012118No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG5030.1160885319301161No Hit
GATGCAGCTTCCAACTCTGCAAACCGTCAGG5030.1160885319301161No Hit
AACTATATCGACTCCGTTCTGAACGAGGTTC4900.11308823190011308No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG4720.10893397032010893No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC4710.1087031780101087No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA4710.1087031780101087No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4680.10801080108010801No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG4610.1063952549101064No Hit
AAAGAGCTGTACGATTCCCCGTCTGCGCAGC4480.10339495488010339No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC4470.10316416257010316No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG4440.10247178564010248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)