FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007927670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442956
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63421.431744913716035No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT53421.2059888566810248No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC27960.6312139354698886No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG27440.6194746205040682No Hit
CGTTCTCGCCACGAGAACACCTCTCAGGTTC21860.4935027406785324No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17670.39891095278086314No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15660.35353398531682606No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC13700.30928579813796403No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG13200.2979979952862135No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12990.2932571180884783No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12120.2736163411264324No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT10620.23975293257118088No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG9860.2225954722365201No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9650.21785459503878488No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9000.20318045133150922No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8910.20114864681819414No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA8650.19527898933528387No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG8560.19324718482196876No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC8450.19076386819458366No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG8150.18399118648353335No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8080.1824108940842883No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8050.18173362591318323No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC8010.1808306016850432No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7890.17812152900062309No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG7620.1720261154606778No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7380.16660797009183756No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC7140.1611898247229973No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7040.15893226415264722No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC6640.1499020218712468No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC6260.14132329170391641No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5960.1345506099928661No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT5630.12710066011071078No Hit
CGTCACGCACATCGCCAGGGTGTAGGCGGCA5520.12461734348332566No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5450.12303705108408058No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5340.12055373445669547No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5140.11603861331599527No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5060.1142325648597152No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC4990.11265227246047012No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC4930.11129773611826005No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4920.11107198006122504No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4510.10181598172278963No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG4480.10113871355168459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)