FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007927706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575946
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT59021.024748847982276No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33430.5804363603532275No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC32710.567935188368354No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC21810.3786813347084622No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG21130.3668746722783039No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19710.3422195830859143No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG19440.3375316435915867No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG19130.33214919454254394No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18780.3260722359387859No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17570.30506332190865115No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16210.2814499970483344No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG14350.24915530275407766No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14110.24498824542578646No Hit
GAAGTCTCCATTGCCGAACCGACCACTTACA13170.22866727088997926No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC13130.2279727613352641No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12700.22050678362207568No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC12180.21147815941077808No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC11590.2012341434787289No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11250.19533081226364973No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10660.18508679633160052No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10340.17953071989387895No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9790.16998121351654497No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8650.15018769120716177No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA8640.15001406381848298No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8490.147409652988301No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8410.1460206338788706No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8300.1441107326034038No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8130.1411590669958642No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7980.13855465616568222No Hit
TCCATCTCTAGCAGCGAGGAAATCGTCCCAA7910.1373392644449306No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG7800.1354293631694638No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7770.1349084810034274No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7540.130915051063815No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7410.12865789501099062No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7400.1284842676223118No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA7200.1250117198487358No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7130.12379632812798422No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7060.12258093640723261No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6820.11841387907894144No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG6740.11702485996951102No Hit
TCCGAGCGTTATCTGGGTTACCTGGAACAGC6720.11667760519215344No Hit
AACGCTACCGTAGCCCTGCAGGGCGAGGTTC6120.10625996187142546No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6070.10539182492803145No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG6020.10452368798463746No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG5920.10278741409784946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)