FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007927739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527667
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT24360.4616547936482668No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA21020.3983572973106145No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG18170.3443459606153123No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA17960.3403661779114479No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT16400.3108020778255983No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG15070.28559678736779065No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG13750.26058101037207176No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT12250.23215399105875487No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA11630.2204041564092505No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC10890.20638016021468086No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG10210.19349324479264385No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG10080.1910295697854897No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT9800.1857231928470039No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG9560.18117486975687316No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8990.17037260241781274No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT8980.17018308895572395No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA8160.15464298506444404No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA8150.15445347160235526No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA7960.15085271582266846No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA7640.14478828503582752No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT7610.1442197446495612No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG7530.14270363695285093No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA7260.1375867734764539No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG7180.13607066577974367No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG7170.13588115231765488No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA6910.13095380230334663No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6870.1301957484549915No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA6760.12811110037201492No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG6560.12432083113023934No Hit
CACGTTCTGAATCGGGCGGGATTGGGTCTGACGACCTTCGATGT6480.1228047234335291No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6250.11844591380548716No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6190.1173088330329545No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA6170.11692980610877694No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG6090.1154136984120667No Hit
GTAAGACAGCACCTGGCCCGGATAGATCAGGTTGGCGTTGGAGA6070.11503467148788914No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6070.11503467148788914No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT5290.10025262144496434No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG5280.10006310798287557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)