FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007927740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527667
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75481.4304476118461074No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT45650.8651289544352783No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT24280.46013868595155655No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC19720.3737205472390731No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG18580.35211601256095226No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG18380.34832574331917665No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG16980.3217938586267476No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC15150.2871128950645009No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG14060.26645592769682397No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12700.2406820968527499No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA12180.2308273968241334No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11200.21225507753943304No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC10700.20277940443499404No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10590.20069475635201747No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA9740.1845861120744712No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9080.17207822357661176No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8570.162413037010084No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA8390.159001794692486No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA8070.15293736390564505No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7990.1514212562089348No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA7670.14535682542209385No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC7570.14346169080120605No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7560.1432721773391173No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7480.14175606964240706No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7390.14005044848360804No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT7260.1375867734764539No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG7000.13265942346214563No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG6750.12792158690992617No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6530.123752290743973No Hit
ATGAACGCACCGGATGATCTGGTCCTGTTCG6380.1209095888126413No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT6300.11939348111593108No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6260.11863542726757595No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6260.11863542726757595No Hit
AAAATTTCCGGCAGCCAGGCATCCTCCCACA6190.1173088330329545No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6180.11711931957086573No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5910.11200245609446868No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5600.10612753876971652No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5560.10536948492136139No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5430.10290580991420727No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5290.10025262144496434No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)