FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007927821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007927821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510520
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG17670.34611768393011044No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC14520.2844158896811094No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG13720.26874559272898224No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG13020.2550340828958709No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT12640.24759069184361043No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG11020.21585834051555275No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9610.1882394421374285No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9090.1780537491185458No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT8920.17472381101621876No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG8870.1737444174567108No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG8600.16845569223536788No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG8230.16120817989500902No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA7600.1488678210452088No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7540.14769254877379925No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA7170.14044503643344042No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7160.14024915772153881No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA6370.12477473948131317No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6350.12438298205751No Hit
GGTACGCTCGACGTGGCAACGTGCCAGCAGCAGACGCAGACCTT6010.1177231058528559No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5770.11302201676721772No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT5420.10616626185066205No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5280.1034239598840398No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG5160.1010734153412207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)