FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007929748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489962
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA30410.6206603777435802No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA28680.5853515170564247No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC25680.5241222788706063No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT25470.519836232197599No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG21400.4367685657255052No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT21200.4326866165131173No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA19310.3941121964560517No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG17030.34757797543482966No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG15560.3175756487237786No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG15460.3155346741175846No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA15340.3130855045901519No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT13010.26553079626583287No Hit
GCGCAGACCCGGGAAACGGTCACGTGCCGCCTGCAGTGCGCGAG12490.25491772831362436No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA12370.25246855878619157No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT12030.24552924512513213No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT11990.24471285528265457No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT11990.24471285528265457No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA11020.22491540160257326No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA9840.20083190124948463No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG9210.18797376123046275No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC8300.16940089231409783No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC7750.1581755319800311No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7680.15674684975569533No Hit
GCGCTGTTTAGCCAGCAGTTCCGCTGCTTTACGTTCTTCCTGCT7510.15327719292516565No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT7350.15001163355525532No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7300.14899114625215834No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG6930.14143954020924074No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6650.13572481131189765No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC6640.13552071385127826No Hit
ACGGTGCGCACGGGTGATAACGTCGTGGTCATCGGAACGGTCGT6570.1340920316269425No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6570.1340920316269425No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6390.1304182773357934No Hit
GTCGCCCGGCAGCGGTGCAGTCGGAGCCCACGGGGAGTTACCGT5910.12062159922606241No Hit
ACGCATTGCGCCGTTAGACGGGTTCAGATAGTACCAAGTGGAAC5720.11674374747429393No Hit
AGTGGTGTGACCCAGGCTTACAACGGAGCCGGTATCGGAAGAAT5670.11572326017119695No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5570.113682285565003No Hit
GGTATCGGAAGAGTCGTCATCCTCTTCTTCGGAGTCTTCTACCG5530.11286589572252542No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5380.10980443381323449No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC5370.1096003363526151No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG5330.10878394651013751No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC5220.10653887444332419No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5150.10511019221898842No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC5070.10347741253403325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)