FastQCFastQC Report
Wed 31 May 2023
EGAF00007929749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489962
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT68321.3943938509517064No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC34570.7055649213612484No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG25920.5290206179254717No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG22760.464525820369743No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG21050.42962515460382644No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA20600.42044076887595366No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT17290.3528845094109339No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG16930.3455370008286357No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG16660.340026369391912No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT15530.3169633563419204No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13770.2810422032729069No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13240.27022503786007895No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13100.2673676734114074No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC13070.2667553810295492No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG12750.2602242622897286No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12600.2571628003804377No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12230.24961119433752005No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA10640.21715969809903624No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9940.2028728758556786No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9590.19572946473399977No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC9390.19164751552161188No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8920.18205493487250032No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8390.1712377694596724No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8250.16838040501100085No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8050.16429845579861296No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG7980.1628697735742772No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7800.15919601928312807No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7640.15593045991321774No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7610.15531816753135957No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7420.15144031577959108No Hit
CATTCTAACGATCAGCACGCGGGTGATCACA7290.14878704879153895No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA7030.14348051481543467No Hit
GCTATGCAGACCGGTTGGTACAAAGTAGGCA5940.12123389160792061No Hit
AGCACCCCGCGTGCGGCTGTCACCCAGACGG5900.12041750176544302No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5820.11878472208048789No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5780.1179683322380103No Hit
TTCGAACAGCCGACCCTGACCTTCGGCGCGG5660.11551916271057756No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5600.11429457794686118No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5480.11184540841942844No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5320.10857984904951812No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC5180.1057224846008466No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5180.1057224846008466No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5070.10347741253403325No Hit
AGCAACAAAACCGAAACTCCGTCCGAGACTG5030.10266102269155568No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG5030.10266102269155568No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5000.10204873030969748No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)