FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007929753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540007
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT36160.6696209493580639No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29140.5396226345214044No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT28670.5309190436420269No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17150.3175884757049446No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC15900.29444062762149376No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15450.2861074023114515No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG14380.26629284435201767No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG14270.264255833720674No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13740.2544411461332909No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG13250.24536718968457819No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG11830.2190712342617781No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC11600.21481203021442313No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA11440.21184910565974144No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11340.2099972778130654No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA11270.20870099832039213No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC11220.2077750843970541No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG10980.20333069756503155No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10540.19518265503965687No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9850.18240504289759207No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9180.16999779632486245No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC9000.16666450620084555No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8760.162220119368823No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8640.1599979259528117No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG8450.1564794530441272No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8130.1505536039347638No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC8090.14981287279609337No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7910.14647958267207647No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7880.14592403431807366No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG7870.14573885153340604No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC7810.1446277548254004No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG7750.14351665811739478No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG7750.14351665811739478No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7550.13981300242404265No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7420.13740562622336377No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7420.13740562622336377No Hit
GCGGAGCAGCACACGCCGATGAAGGCGCACG7160.13259087382200602No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG6890.12759093863598064No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG6540.12110954117261442No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG6510.12055399281861161No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6340.11740588547926231No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6110.11314668143190736No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5850.10833192903054961No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5710.10573937004520312No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5630.10425790776786227No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG5620.10407272498319466No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC5510.10203571435185099No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)