FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007929761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490202
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT109362.2309170505220295No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49951.0189676908702943No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT24420.498161982203255No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG22780.4647063863468529No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20750.42329488659776987No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC19520.39820318970546836No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG19040.38841130799139945No Hit
GCTTCCGAACCGGAGGACAAATCTCCGCGCG18780.3831073720629455No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA17230.35148775402793137No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC13810.28172059681519046No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13360.2725407077082509No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG12010.2450010403874321No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG11830.24132908474465628No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10670.21766537060232313No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10220.20848548149538354No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9750.19889759731702442No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9640.19665362442421697No Hit
GCAAGCGAACCGGAGGACAAATCTCCGCGCG9300.18971770821008482No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA9250.18869772053153597No Hit
ATGATGGCTCGTCGCACCGTTGATTTCAAAA9060.1848217673530504No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA8880.18114981171027453No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8810.17972182896030614No Hit
TTCGAACAGCCGACCCTGACCTTCGGCGCGG8760.1787018412817573No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT8080.16483000885349305No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC8080.16483000885349305No Hit
GAACCGGAAGACAAATCCCCGCGTGTTCAGC7330.1495301936752604No Hit
CAGTCCATTGTAAACCGTACTGACCTGGATC7190.14667422817532363No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG7170.1462662331039041No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA6990.14259427746112827No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA6480.13219040313993008No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA6360.12974243271141284No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6330.12913044010428354No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6330.12913044010428354No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5910.12056254360447326No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA5860.11954255592592442No Hit
CCGCGTGTCCTGAGCAACTCCGGTTGGGGTC5770.1177065781045365No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5710.11648259289027789No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA5700.11627859535456811No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA5650.11525860767601928No Hit
CCGCCGCGCACTCCAACCTGGGAATCTCCGG5600.11423861999747044No Hit
TACTATAACGGTAAAGTTCCGTATGATGCAC5480.11179064956895321No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5470.11158665203324343No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5330.10873068653330668No Hit
CGTATCCGTCGCGAAACCGAAGAAAAAGAAG5140.10485473335482107No Hit
CAGACCCCAGAACAGTCTACCCCGTCTCGCA5010.10220276539059409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)