FastQCFastQC Report
Wed 31 May 2023
EGAF00007929763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495529
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT108582.191193653650947No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44370.8954067269524085No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT24250.48937600019373234No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG23960.48352366864502383No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21020.4241931350132888No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG19920.4019946360354288No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC19360.3906935820103364No Hit
GCTTCCGAACCGGAGGACAAATCTCCGCGCG18740.37818170076826985No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA18230.3678896694239893No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC14210.28676424588671906No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13630.275059582789302No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG11960.24135822525018719No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG11710.23631311184612808No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10690.21572904915756697No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC10150.20483160420479932No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA10050.20281355884317567No Hit
GCAAGCGAACCGGAGGACAAATCTCCGCGCG9870.19918107719225311No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9800.19776844543911656No Hit
ATGATGGCTCGTCGCACCGTTGATTTCAAAA9740.19655761822214238No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9370.18909085038413492No Hit
TTCGAACAGCCGACCCTGACCTTCGGCGCGG9340.18848543677564786No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA9160.1848529551247253No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8720.1759735555335813No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC8160.1646725015084889No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT7690.15518768830885782No Hit
CAGTCCATTGTAAACCGTACTGACCTGGATC7140.1440884388199278No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC7000.1412631753136547No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG6980.14085956624132998No Hit
GAACCGGAAGACAAATCCCCGCGTGTTCAGC6910.13944693448819342No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA6850.13823610727121927No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA6650.13420001654797198No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA6410.12935670768007523No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6110.12330257159520432No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA6050.12209174437823013No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA6010.12128452623358069No Hit
CCGCCGCGCACTCCAACCTGGGAATCTCCGG6000.12108272169741832No Hit
TACTATAACGGTAAAGTTCCGTATGATGCAC5990.12088091716125596No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA5940.11987189448044414No Hit
CCGCGTGTCCTGAGCAACTCCGGTTGGGGTC5880.11866106726346995No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5770.11644121736568394No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5750.11603760829335921No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5470.11038708128081304No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5400.10897444952767649No Hit
AACATCTTTACCCCGATCAAGAAACCGGGCA5370.1083690359191894No Hit
CGTATCCGTCGCGAAACCGAAGAAAAAGAAG5100.10292031344280557No Hit
ACCCTGGAACCGGTATCCCGCATTCGTTCTC5000.10090226808118193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)