FastQCFastQC Report
Sun 21 May 2023
EGAF00007929767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470788
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT96092.041046075940763No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41970.8914840650144014No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29690.6306447912860991No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT24710.5248646949369993No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC20100.42694376237287274No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20060.42609412304476746No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18210.38679830411990107No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG18120.3848866156316644No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG17150.36428286192511283No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14530.3086314859342209No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14230.3022591909734318No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12890.27379627348190694No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12880.27358386364988063No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA12780.2714597653296176No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11820.2510684214550923No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC10360.22005658597925182No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9900.21028573370604178No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG8960.19031920949556913No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA8560.18182281621451693No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7960.16907822629293864No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7910.16801617713280712No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7460.15845773469162341No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7410.15739568553149189No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7200.1529350790589395No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7190.1527226692269132No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG7170.15229784956286055No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6740.14316422678572946No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6720.14273940712167685No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG6710.14252699728965054No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT6610.14040289896938749No Hit
CTGCGTTTCAAAACCGGCCGTAACGCTGCGT6360.13509265316872987No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6270.13318096468049312No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6120.12999481720009856No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6080.12914517787199334No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA5950.12638385005565136No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5940.1261714402236251No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5770.12256047307917788No Hit
ATGGGTGCACAGGTTTCCACTCAGAAATCTG5720.12149842391904636No Hit
GACGCAGCTTCCTCTGGTGCGAGCCGTCTGG5690.12086119442296746No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5510.11703781744649396No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5360.11385166996609938No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5250.11151516181381003No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA4970.10556768651707349No Hit
AACCTGACCGCGGAACTGGATAAAGTTAAAG4740.1006822603804685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)