FastQCFastQC Report
Wed 8 May 2024
EGAF00007929769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462893
Total Bases14.3 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT75791.6373114305033778No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29410.6353520143964156No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA19670.42493621636101647No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19100.4126223554903617No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18770.40549327814419317No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG15580.33657886379789714No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14990.32383293763353516No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14870.3212405458712921No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG13620.294236465014593No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12740.2752255920914769No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG12220.26399189445509No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG11400.24627721741309547No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11030.23828400947951256No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG10250.22143346302493233No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA10160.21948916920324998No Hit
CGTTCTCGCCACGAGAACACCTCTCAGGTTC9690.20933563480113113No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9260.20004623098642668No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG9110.1968057412836228No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8940.1931331862871117No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8940.1931331862871117No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8680.1875163374689183No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8570.18513997835352877No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG7880.1702337257206309No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG7720.16677720337097343No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA7660.16548100748985187No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7650.16526497484299826No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6970.15057475485695399No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG6910.14927855897583245No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6800.1469021998604429No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6710.14495790603876058No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG6290.1358845348709097No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG6250.13502040428349532No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6030.13026768605271627No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5980.12918752281844834No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5970.12897149017159473No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5890.127243228996766No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5790.12508290252823007No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5650.12205844547227976No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA5540.11968208635689025No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5400.11665762930093997No Hit
CGTGGCCAGAGCCGTGGCCGTGGCCGTGGTC5380.11622556400723277No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT5350.11557746606667199No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA5240.11320110695128248No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5050.10909648666106422No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4910.10607202960511393No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG4910.10607202960511393No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG4760.10283153990231003No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4750.10261550725545646No Hit
GTTATTTCTCTGACTCGTCGCACCCGTCAGA4740.10239947460860285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)