FastQCFastQC Report
Wed 31 May 2023
EGAF00007929775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440307
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT46791.0626676387157143No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC32450.7369857849182503No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG26600.6041239407958537No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA20740.47103498241000025No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG18860.4283375008800678No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16820.3820061911348218No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG15900.36111167889676976No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15820.35929476478911304No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG13160.29888237070952767No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12890.29275028559618627No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG12500.28389282932135984No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10310.23415480562425764No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG10190.2314294344627726No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9650.2191652642360898No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9400.2134874076496626No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9280.21076203648817757No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8800.19986055184223736No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC8800.19986055184223736No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT8550.19418269525581014No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT8290.18827772440592586No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8010.18191852502912742No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG7740.17578643991578602No Hit
ATGTCCAACATGGACATCGATGGTATTAACA7670.17419664007158642No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7610.17283395449084388No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7210.1637493839525604No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6860.1558003847315623No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG6610.1501225281451351No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5680.12900090164362593No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5670.12877378738016884No Hit
ATGGGTGCAGACATGGTAGAACTGGACATTC5630.12786533032634048No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5510.12513995916485543No Hit
ACCCGCCACATTGACGCAAAAGCAGTTTACG5320.12082478815917076No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5190.11787230273422862No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG4890.11105887483051598No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA4580.10401833266334626No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC4510.10242853281914664No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG4470.1015200757653183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)