FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007929790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414314
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT38680.933591430654045No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT34930.8430803689954962No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT23700.5720299096820286No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT22870.5519967947016031No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC21820.5266536974372095No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT20610.49744879487538435No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT19090.4607616445497859No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG17690.426970848197261No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG16460.397283219973257No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC15600.37652601649956313No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG14700.35480336170151144No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA14650.35359654754606407No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG14250.34394203430248554No Hit
AACGTGACGGGTCTGAACGGTGTCAGACGGGGCCAGCGGGTTGG13070.31546122023392886No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG12230.29518674242241394No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG12040.2906008486317141No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG11270.27201591063782543No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT10980.26501638853623094No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10750.25946504342117327No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT10680.25777550360354706No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC10620.2563273266170103No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA10360.25005189300868425No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10020.2418455567516425No Hit
CTGAACGACGTGACGGGTCTGTACAGTGTCAGACGGGGCCAGCG10010.241604193920553No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG9910.2391905656096584No Hit
GGAGCCCAGGCTGTCGAACGCTGGGTGCAGCAGAGCCAGACCTT9840.23750102579203214No Hit
TACGTGACGGGTCTGCACGGTGTCAGACGGCGCCAGCGGGTTGG9530.23001877802825876No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT9110.2198815391225013No Hit
CTGCACTACGTGACGGGTCTGGACGGTATCAGACGGCGCCAGCG8860.2138474683452647No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8810.2126406541898174No Hit
CTGGACTACGTGACGCGTCTGAACGGTATCGCTCGGCGCCAGCG8120.1959866188446444No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC8100.19550389318246547No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA7880.19019391089849727No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT7690.18560801710779748No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT7580.18295302596581337No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG7560.18247030030363445No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG7560.18247030030363445No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7440.17957394633056087No Hit
CACGCTAGACTGCTCGGACGGACCTGGACGTTCTGGGGTAGACT7350.17740168085075572No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6790.16388536230974576No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC6730.16243718532320897No Hit
TGCCCCATTTAAATCAGCCTGTCACTGGACAACGTGACGGGTCT6680.16123037116776165No Hit
AACGCTAGACTGTTCGCTCGGACCAGGACGTTCTGGAGTGGACT6400.15447221189725668No Hit
GTTGGTAGCGCCGGTTTCCACCGCGGTCAGCGCCGGAACTTCTT6180.14916222961328846No Hit
AGTGGTTTTGTGAACGATACGGACCTTCTGCGGCACGCCACGCA6150.14843814112002007No Hit
TGCATCTTCGAAGTCTTCATCATCGTCGTCGTCTTCTTCTTCAC5990.14457633582258866No Hit
CGGCGAAGCGACCGTTGAAAAAGGTGGATGCAACTGATTTAAAT5910.14264543317387296No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5900.1424040703427835No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5670.13685272522772585No Hit
CGTACTCCTGGCGACAGAGAAAATGGCGGTGACGTTATTTAAAT5590.13492182257901011No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5100.12309504385562642No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5100.12309504385562642No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA4830.11657824741621088No Hit
CCCATGACGGATTCCTGTCACCGAAGGATGCGTACTCCTGGCGA4680.11295780494986894No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG4580.11054417663897431No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4570.11030281380788483No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC4530.10933736248352698No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC4520.10909599965243752No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG4420.10668237134154289No Hit
GTAACCCTGCGCGATTTTAATCTGAGAGACACCGTATTTATCGG4380.10571692001718504No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4340.10475146869282718No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA4170.10064830056430629No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)