FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007929791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007929791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414314
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60811.4677273758550278No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA37130.8961801918351782No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT36590.8831465989563472No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT34190.8252195194948759No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC26660.6434733076845098No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG23600.569616281371134No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA22050.5322050425522671No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC21130.5099996620920364No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20140.48610474181417956No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG17680.42672948536617156No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC17120.4132131668251616No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA16460.397283219973257No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16240.3919732376892888No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG15870.3830428129389786No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC15540.3750778395130263No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC14350.3463556626133802No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA13270.32028847685571815No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA12860.31039260078105013No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG12700.30653079548361867No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA11960.2886699459829984No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC11170.2696022823269308No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG11100.2679127425093045No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT10830.26139594606988903No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10790.26043049474553115No Hit
GCGTACGCACTGTCTTCTCAGCAGAAAGCAC10770.25994776908335226No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG10680.25777550360354706No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10550.25463778679938404No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG10330.24932780451541584No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC10200.24619008771125284No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT10100.2437764594003582No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG9800.23653557446767426No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9530.23001877802825876No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9230.22277789309557483No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC9110.2198815391225013No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC8950.21601973382506987No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8860.2138474683452647No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG8570.20684794624367026No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG8540.20612385775040185No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT7800.18826300824978157No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG7750.18705619409433424No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA7410.1788498578372925No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6870.16581626495846144No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT6760.16316127381647735No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6710.16195445966103003No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6310.15229994641745148No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT6250.15085176943091472No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT6120.1477140526267517No Hit
GCAAACCCGCTGCACGAAGAATTTCGTGCCC6110.14747268979566222No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5750.13878362787644152No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5370.12961184029504194No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5350.129129114632863No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC5180.12502594650434212No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4990.1204400527136423No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC4930.11899187572710553No Hit
CTGGTGGACTACCAGGATAAGATGATCAAAG4830.11657824741621088No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4810.11609552175403197No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4440.10716509700372183No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4380.10571692001718504No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA4180.10088966339539578No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4170.10064830056430629No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)